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Targeted Allele Detail
Symbol: Vpreb2tm3Ilm
Name: pre-B lymphocyte gene 2; targeted mutation 3, Inga-Lill Martensson
MGI ID: MGI:3842838
Gene: Vpreb2  Location: Chr16:17980428-17981273 bp, + strand  Genetic Position: Chr16, 11.14 cM
Germline Transmission:  Earliest citation of germline transmission: J:67609
Parent Cell Line:  Other (see notes) (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA single copy of a construct in which the entire coding region was replaced with a hygromycin cassette was incorporated at the endogenous locus via homologous recombination. Insertion occurred trans to the pre-existing mutant allele. Transcript was undetected by RT-PCR analysis of bone marrow cells obtained from homozygous mutant mice. The absence of protein surface expression was confirmed by FACS analysis. (J:67609)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Vpreb2 Mutation:  3 strains or lines available
ES cells heterozygous for Vpreb1tm1Ilm were electroporated with the targeting vector. Three targeted mutant lines identified as ES47, ES65, and ES223 were found in which targeted insertion occurred trans to the pre-existing mutant allele.
Original:  J:67609 Mundt C, et al., Loss of precursor B cell expansion but not allelic exclusion in VpreB1/VpreB2 double-deficient mice. J Exp Med. 2001 Feb 19;193(4):435-45
All:  2 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
The Jackson Laboratory