Vpreb1b^tm3Ilm
Targeted Allele Detail

Summary

• Symbol: Vpreb1b^tm3Ilm
• Name: V-set pre-B cell surrogate light chain 1B; targeted mutation 3, Inga-Lill Martensson
• MGI ID: MGI:3842838
• Gene: Vpreb1b Location: Chr16:17798292-17799137 bp, + strand Genetic Position: Chr16, 11.14 cM
• Alliance: Vpreb1b^tm3Ilm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67609
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A single copy of a construct in which the entire coding region was replaced with a hygromycin cassette was incorporated at the endogenous locus via homologous recombination. Insertion occurred trans to the pre-existing mutant allele. Transcript was undetected by RT-PCR analysis of bone marrow cells obtained from homozygous mutant mice. The absence of protein surface expression was confirmed by FACS analysis. (J:67609)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vpreb1b Mutation: 8 strains or lines available

Notes

ES cells heterozygous for Vpreb1^tm1Ilm were electroporated with the targeting vector. Three targeted mutant lines identified as ES47, ES65, and ES223 were found in which targeted insertion occurred trans to the pre-existing mutant allele.

References

• Original: J:67609 Mundt C, et al., Loss of precursor B cell expansion but not allelic exclusion in VpreB1/VpreB2 double-deficient mice. J Exp Med. 2001 Feb 19;193(4):435-45
• All: 2 reference(s)