Cdkn2a^tm4Cjs
Targeted Allele Detail

Summary

• Symbol: Cdkn2a^tm4Cjs
• Name: cyclin dependent kinase inhibitor 2A; targeted mutation 4, Charles J Sherr
• MGI ID: MGI:3842647
• Synonyms: Arf^FL, Arf^LoxP, Cdkn2a^flx
• Gene: Cdkn2a Location: Chr4:89192710-89212856 bp, - strand Genetic Position: Chr4, 42.15 cM, cytoband C3-C6
• Alliance: Cdkn2a^tm4Cjs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147752
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The first coding exon (1Beta) is flanked by loxP sites. A loxP-neoTK-loxP cassette was inserted downstream of the floxed exon and was excised in the targeted ES cells. Cell clones with the selection cassette removed were used to generated mouse lines with a conditional Cdkn2a allele. (J:147752)

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Cdkn2a^tm4Cjs

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Cdkn2a Mutation: 62 strains or lines available

References

• Original: J:147752 Gromley A, et al., Transient expression of the Arf tumor suppressor during male germ cell and eye development in Arf-Cre reporter mice. Proc Natl Acad Sci U S A. 2009 Apr 14;106(15):6285-90
• All: 14 reference(s)