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Seletm2Hyn
Targeted Allele Detail
Nomenclature
Symbol: Seletm2Hyn
Name: selectin, endothelial cell; targeted mutation 2, Richard Hynes
MGI ID: MGI:3839963
Synonyms: E-selectin-
Gene: Sele  Location: Chr1:164048234-164057677 bp, + strand  Genetic Position: Chr1, 71.35 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:31626
Parent Cell Line:  D3 (ES Cell)
Strain of Origin:  129S2/SvPas
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA modified D3 ES cell line heterozygous for the Selptm1Hyn allele was targeted a second time. The exons encoding the signal peptide, lectin domain, and a portion of the epidermal growth factor domain were replaced by the insertion of a PGK-hygro cassette. Both Northern and RT-PCR analysis revealed an absence of normal mRNA for E-selectin but the presence of mRNA for P-selectin in extracts from cardiac and pulmonary tissue of homozygous mutant mice. This targeted mutation occurred in trans with the Selptm1Hyn mutation and were subsequently bred apart. (J:31626)
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 4 strains available      Cell Lines: 0 lines available
Carrying any Sele Mutation:  25 strains or lines available
References
Original:  J:31626 Frenette PS, et al., Susceptibility to infection and altered hematopoiesis in mice deficient in both P- and E-selectins. Cell. 1996 Feb 23;84(4):563-74
All:  14 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
08/17/2016
MGI 6.05
The Jackson Laboratory