Tg(Thy1-Cerulean/TNNC*/Venus)CAkon
Transgene Detail

Summary

• Symbol: Tg(Thy1-Cerulean/TNNC*/Venus)CAkon
• Name: transgene insertion C, Arthur Konnerth
• MGI ID: MGI:3839673
• Synonyms: CerTn-L15, Tg(Thy1-CFP*/TNNC*/YFP*)CAkon
• Transgene: Tg(Thy1-Cerulean/TNNC*/Venus)CAkon Location: unknown
• Alliance: Tg(Thy1-Cerulean/TNNC*/Venus)CAkon page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The Thy1 promoter was used to drive expression of a fluorescent fusion construct. The three part fusion proein is composed of "cerulean" which is a modified CFP encoding S72A and Y145A amino acid substitutions, chicken troponin C truncated to start with sequence encoding amino acid residue 15, and "citrine" which is a modified EYFP encoding Q69M, C48L, andC70V amino acid residue substitutions. "Citrine" was further modified to encode V163A and S175G substitutions. (J:147189)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:147189 Heim N, et al., Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor. Nat Methods. 2007 Feb;4(2):127-9
• All: 2 reference(s)