Tg(Clgn-Adam3)47Osb
Transgene Detail

Summary

• Symbol: Tg(Clgn-Adam3)47Osb
• Name: transgene insertion 47, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:3838539
• Synonyms: Adam3 tg#47 (His+), Tg(Clgn-Adam3/His)47Osb
• Transgene: Tg(Clgn-Adam3)47Osb Location: unknown
• Alliance: Tg(Clgn-Adam3)47Osb page

Transgene origin

• Strain of Origin: 129S1/Sv and 129X1/SvJ and CD-1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Clgn-Adam3)47Osb expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Adam3 (MGI:102518)

• Tg(Clgn-Adam3)47Osb expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Clgn (MGI:107472)

• Mutation details: This transgene comprises a full length cDNA encoding C-terminal polyhistidine-tagged mouse ADAM3 [a disintegrin and metallopeptidase domain 3 (cyritestin)] followed by a bovine globin polyadenylation signal and expressed under control of the testis specific promoter of the mouse calmegin gene. Western blot analysis demonstrated the presence of ADAM3 in both testis and sperm of homozygous Adam3^tm1Ihig mice bearing this transgene. (J:147330)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

The transgene construct was microinjected into fertilized oocytes heterozygous for Adam3^tm1Ihig on a mixed strain (129S1/Sv, 129X1/SvJ and CD-1) background.

References

• Original: J:147330 Yamaguchi R, et al., Disruption of ADAM3 impairs the migration of sperm into oviduct in mouse. Biol Reprod. 2009 Jul;81(1):142-6
• All: 1 reference(s)