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Gt(ROSA)26Sortm1(CAG-Kcnj11*,-GFP)Nich
Targeted Allele Detail
Nomenclature
Symbol: Gt(ROSA)26Sortm1(CAG-Kcnj11*,-GFP)Nich
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Colin G Nichols
MGI ID: MGI:3838361
Synonyms: mutKir6.2, Rosa26-Kir6.2, Rosa26-Kir6.2(K185Q,deltaN30)
Gene: Gt(ROSA)26Sor  Location: Chr6:113067428-113077333 bp, - strand  Genetic Position: Chr6, 52.73 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:146650
Parent Cell Line:  v6.5 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Mutation
description
Allele Type:    Targeted (Conditional ready, Inserted expressed sequence, Reporter)
Mutation:    Insertion
 
Gt(ROSA)26Sortm1(CAG-Kcnj11*,-GFP)Nich expresses 1 gene
 
Mutation detailsThe targeting construct contained the CAG promoter (chicken beta actin promoter with CMV enhancers), a floxed neomycin-Westphal stop sequence (WSS) cassette, a modified Kcnj11 gene, an IRES and GFP sequence. Kcnj11 has been modified by a 30 amino acid N-terminal truncation and by a modification of the codon for amino acid residue 185 causing a lysine to glutamine substitution. Cre mediated excision of the neo-WSS cassette permits expression of the mutated Kcnj11 and GFP under the control of the CAG promoter. (J:146650)
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  280 strains or lines available
References
Original:  J:146650 Remedi MS, et al., Secondary consequences of beta cell inexcitability: identification and prevention in a murine model of K(ATP)-induced neonatal diabetes mellitus. Cell Metab. 2009 Feb;9(2):140-51
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
04/07/2015
MGI 5.21
The Jackson Laboratory