Ttr^tm1Kymm
Targeted Allele Detail

Summary

• Symbol: Ttr^tm1Kymm
• Name: transthyretin; targeted mutation 1, Ken-ichi Yamamura
• MGI ID: MGI:3838181
• Synonyms: Ttr^-
• Gene: Ttr Location: Chr18:20798337-20807378 bp, + strand Genetic Position: Chr18, 11.47 cM
• Alliance: Ttr^tm1Kymm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:146510
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout, RMCE-ready)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A null allele was created by replacing the ATG start site within exon 1 with a PGK-neo cassette flanked by lox71 and loxP sites. Besides disrupting the gene, the cassette also serve as an acceptor in recombination mediated cassette exchange. No protein was detected in immunoblot analysis of homozygote livers. (J:146510)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ttr Mutation: 44 strains or lines available

References

• Original: J:146510 Zhao G, et al., Inconsistency between hepatic expression and serum concentration of transthyretin in mice humanized at the transthyretin locus. Genes Cells. 2008 Nov 13;13(12):1257-1268
• All: 2 reference(s)