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Mcl1tm1Yen
Targeted Allele Detail
Nomenclature
Symbol: Mcl1tm1Yen
Name: myeloid cell leukemia sequence 1; targeted mutation 1, Hsin-Fang Yang-Yen
MGI ID: MGI:3837368
Synonyms: Mcl-1SC
Gene: Mcl1  Location: Chr3:95658721-95663176 bp, + strand  Genetic Position: Chr3, 40.74 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:146246
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsTwo upstream promoter elements located at positions -87 (the SIE site) and -70 (the CRE-2 site) were targeted for mutation. The SIE and CRE-2 sites were replaced with BamHI and EcoRI restriction site, respectively, and a floxed neomycin resistance cassette was placed downstream of exon 1. Founder mice were crossed with EIIa-Cre transgenic mice to remove the neomycin cassette. Immunoblot analysis revealed gene expression was reduced by 90% in the thymus but was marginally or not at all affected in various other major organs examined, including liver, heart, lung and spleen. The altered expression was attributed to the lack of SIE and CRE-2 sites because expression was not disturbed in the thymus of mice homozygous for the same loxP insertion but with intact promoter elements. (J:146246)
Phenotypes
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Mcl1 Mutation:  36 strains or lines available
References
Original:  J:146246 Yang CY, et al., Promoter knock-in mutations reveal a role of Mcl-1 in thymocyte-positive selection and tissue or cell lineage-specific regulation of Mcl-1 expression. J Immunol. 2009 Mar 1;182(5):2959-68
All:  1 reference(s)

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last database update
07/21/2015
MGI 5.22
The Jackson Laboratory