Tg(Prnp-APOE4)3R1Dbo
Transgene Detail

Summary

• Symbol: Tg(Prnp-APOE4)3R1Dbo
• Name: transgene insertion 3R1, David R Borchelt
• MGI ID: MGI:3835599
• Synonyms: Hu Apo E4, Tg(Prnp-APOE4)3Dbo
• Transgene: Tg(Prnp-APOE4)3R1Dbo Location: unknown
• Alliance: Tg(Prnp-APOE4)3R1Dbo page

Transgene origin

• Strain of Origin: (C3H x C57BL/6J)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Prnp-APOE4)3R1Dbo expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APOE (348)

  Apoe	(MGI:88057)

• Mutation details: A human cDNA encoding the E4 variant of the APOE protein, which contains arginine in place of cysteine at amino acid position 112 (C112R), was inserted into an expression cassette containing the promoter, 5' intronic and 5' and 3' untranslated region sequences of the mouse prion protein gene. Western blot analysis revealed levels of human APOE4 approaching 100 ng/ug of total protein in brains of transgenic mice, approximately five fold that of endogenous mouse APOE. Astroglia and neurons cultured from transgenic mice contain the human protein, the level in neurons approximately five times that in astrocytes; however, secretion by astroglia is much greater. (J:72987)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:72987 Lesuisse C, et al., Hyper-expression of human apolipoprotein E4 in astroglia and neurons does not enhance amyloid deposition in transgenic mice. Hum Mol Genet. 2001 Oct 15;10(22):2525-37
• All: 1 reference(s)