Hprt1^{tm20(Ple53-EGFP)Ems}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm20(Ple53-EGFP)Ems}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 20, Elizabeth M Simpson
• MGI ID: MGI:3835534
• Synonyms: DCX-EGFP, Hprt^{tm20(mEMS1892)Ems}, Ple53
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm20(Ple53-EGFP)Ems} page

Mutation origin

• Mutant Cell Lines: mEMS1886, mEMS1892, mEMS1894, mEMS1913
• Germline Transmission: Earliest citation of germline transmission: J:145689
• Parent Cell Line: mEMS1204 (ES Cell)
• Strain of Origin: (B6.129P2-Hprt1^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
• Project Collection: Pleiades Promoter Project

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The Ple53-EGFP transgene (pEMS1199) was designed with the 3520 bp Ple53 minipromoter (DCX-B; derived from a portion of the promoter of the DCX gene from the doublecortin (DCX) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome. EGFP-immunoreactivity is detected most strongly in the neurogenic cells of the rostral migratory stream (RMS). (J:144244, J:164356)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1273 strains or lines available

Notes

Germ line transmission of mutant cell line mEMS1892 has been established.

References

• Original: J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009
• All: 3 reference(s)