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Targeted Allele Detail
Symbol: Hprttm20(Ple53-EGFP)Ems
Name: hypoxanthine guanine phosphoribosyl transferase; targeted mutation 20, Elizabeth M Simpson
MGI ID: MGI:3835534
Synonyms: DCX-EGFP, Hprttm20(mEMS1892)Ems, Ple53
Gene: Hprt  Location: ChrX:52077014-52110536 bp, + strand  Genetic Position: ChrX, 29.31 cM, cytoband A6
Mutant Cell Lines:  mEMS1886, mEMS1892, mEMS1894, mEMS1913
Germline Transmission:  Earliest citation of germline transmission: J:145689
Parent Cell Line:  mEMS1204 (ES Cell)
Strain of Origin:  (B6.129P2-Hprtb-m3/J x 129S-Gt(ROSA)26Sortm1Sor/J)F1
Project Collection: Pleiades Promoter Project
Allele Type:    Targeted (Reporter)
Mutation:    Insertion
Mutation detailsThe Ple53-EGFP transgene (pEMS1199) was designed with the 3520 bp Ple53 minipromoter (DCX-B; derived from a portion of the promoter of the DCX gene from the doublecortin (DCX) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. EGFP-immunoreactivity is detected most strongly in the neurogenic cells of the rostral migratory stream (RMS). (J:144244, J:164356)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Hprt Mutation:  1274 strains or lines available
Germ line transmission of mutant cell line mEMS1892 has been established.
Original:  J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009;
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.17
The Jackson Laboratory