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Targeted Allele Detail
Symbol: Hprttm12(Ple177-EGFP/cre)Ems
Name: hypoxanthine guanine phosphoribosyl transferase; targeted mutation 12, Elizabeth M Simpson
MGI ID: MGI:3835523
Synonyms: Hprt1tm12(mEMS762)Ems, Hprt1tm12(Ple177-EGFPcre;mEMS762)Ems, RGS16-C-EGFP/cre
Gene: Hprt  Location: ChrX:52988137-53021659 bp, + strand  Genetic Position: ChrX, 29.31 cM, cytoband A6
Mutant Cell Lines:  mEMS761, mEMS762, mEMS763, mEMS765
Germline Transmission:  Earliest citation of germline transmission: J:145689
Parent Cell Line:  E14TG2a (ES Cell)
Strain of Origin:  129P2/OlaHsd
Project Collection: Pleiades Promoter Project
Allele Type:    Targeted (Recombinase, Reporter)
Mutation:    Insertion     Vector Type: Not Applicable
Mutation detailsThe Ple177-EGFPCre transgene (pEMS1089) was designed with the 1302 bp Ple177 minipromoter (RGS16-C; derived from an upstream candidate regulatory regions and a subsection of the human regulator of G-protein signaling 16 (/RGS16/) promoter) upstream of a minimal F5 mutant-/frt/ site, an EGFP/Cre fusion protein (enhanced green fluorescent protein (with mutated stop) and Cre recombinase), a nuclear localization signal, a second minimal /frt/ site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). (J:164356)
 Tissue activity of this recombinase allele
Driver: RGS16     Summary of all recombinase alleles driven by RGS16.

View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Hprt Mutation:  1272 strains or lines available
Germ line transmission of mutant cell line mEMS762 has been established.
Original:  J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.16
The Jackson Laboratory