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Hprttm12(Ple177-EGFP/cre)Ems
Targeted Allele Detail
Nomenclature
Symbol: Hprttm12(Ple177-EGFP/cre)Ems
Name: hypoxanthine guanine phosphoribosyl transferase; targeted mutation 12, Elizabeth M Simpson
MGI ID: MGI:3835523
Synonyms: Hprt1tm12(mEMS762)Ems, Hprt1tm12(Ple177-EGFPcre;mEMS762)Ems, RGS16-C-EGFP/cre
Gene: Hprt  Location: ChrX:52988137-53021659 bp, + strand  Genetic Position: ChrX, 29.31 cM, cytoband A6
Mutation
origin
Mutant Cell Lines:  mEMS761, mEMS762, mEMS763, mEMS765
Germline Transmission:  Earliest citation of germline transmission: J:145689
Parent Cell Line:  E14TG2a (ES Cell)
Strain of Origin:  129P2/OlaHsd
Project Collection: Pleiades Promoter Project
Mutation
description
Allele Type:    Targeted (Recombinase, Reporter)
Mutation:    Insertion     Vector Type: Not Applicable
 
Mutation detailsThe Ple177-EGFPCre transgene (pEMS1089) was designed with the 1302 bp Ple177 minipromoter (RGS16-C; derived from an upstream candidate regulatory regions and a subsection of the human regulator of G-protein signaling 16 (/RGS16/) promoter) upstream of a minimal F5 mutant-/frt/ site, an EGFP/Cre fusion protein (enhanced green fluorescent protein (with mutated stop) and Cre recombinase), a nuclear localization signal, a second minimal /frt/ site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). (J:164356)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: RGS16     Summary of all recombinase alleles driven by RGS16.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Hprt Mutation:  1272 strains or lines available
Notes
Germ line transmission of mutant cell line mEMS762 has been established.
References
Original:  J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
04/06/2021
MGI 6.16
The Jackson Laboratory