Tg(Myh6-Pln)78Egk
Transgene Detail

Summary

• Symbol: Tg(Myh6-Pln)78Egk
• Name: transgene insertion 78, Evangelia G Kranias
• MGI ID: MGI:3834115
• Synonyms: non-phosphorylatable PLB, (S16A,T17A), Ser¹⁶-Thr¹⁷--> Ala-Ala, Tg(Pln)78Egk
• Transgene: Tg(Myh6-Pln)78Egk Location: unknown
• Alliance: Tg(Myh6-Pln)78Egk page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Myh6-Pln)78Egk expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Pln (MGI:97622)

• Mutation details: The transgene contains a mouse cardiac phospholamban cDNA with a point mutation (TCC->GCC) that encodes alanine instead of serine at amino acid position 16 of the protein (S16A) and a second point mutation (ACT->GCT) that encodes alanine instead of threonine at amino acid position 17 of the protein (T17A), followed by an SV40 polyadenylation signal, under control of the cardiac-specific alpha myosin heavy chain (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter. The replaced serine and threonine normally are phosphorylated, independently of one another and by different phosphorylases, in response to stimulation of cardiac muscle beta-adrenergic receptors. Northern blot analysis revealed cardiac expression of the 1.0 kb transcript from the transgene, and quantitative immunoblot analysis demonstrated 1.8 fold the wild-type level of phospholamban protein in the hearts of transgenic mice. (J:144920)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:144920 Brittsan AG, et al., Maximal inhibition of SERCA2 Ca(2+) affinity by phospholamban in transgenic hearts overexpressing a non-phosphorylatable form of phospholamban. J Biol Chem. 2000 Apr 21;275(16):12129-35
• All: 5 reference(s)