Tg(Myh6-Pln)91Egk
Transgene Detail

Summary

• Symbol: Tg(Myh6-Pln)91Egk
• Name: transgene insertion 91, Evangelia G Kranias
• MGI ID: MGI:3834112
• Synonyms: Asn²⁷-->Ala, N27A PLB, PLB N27A, Tg(Pln)91Egk
• Transgene: Tg(Myh6-Pln)91Egk Location: unknown
• Alliance: Tg(Myh6-Pln)91Egk page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Myh6-Pln)91Egk expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Pln (MGI:97622)

• Mutation details: The transgene contains a full length mouse cardiac phospholamban cDNA with two consecutive point mutations (AAT->GCT) that encodes alanine instead of asparagine at amino acid position 27 of the protein (N27A), followed by an SV40 polyadenylation signal, under control of the cardiac-specific alpha myosin heavy chain (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter. Quantitative immunoblot analysis demonstrated cardiac expression of phospholamban protein in this line (91) at 140 +/- 12% the level observed in wild-type hearts. (J:144921)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Two transgenic lines, designated 29 and 91, demonstrated cardiac expression of transgene derived transcripts and overexpression of phospholamban protein. (J:144921)

References

• Original: J:144921 Zhai J, et al., Cardiac-specific overexpression of a superinhibitory pentameric phospholamban mutant enhances inhibition of cardiac function in vivo. J Biol Chem. 2000 Apr 7;275(14):10538-44
• All: 2 reference(s)