Tg(Myh6-Pln)22Egk
Transgene Detail

Summary

• Symbol: Tg(Myh6-Pln)22Egk
• Name: transgene insertion 22, Evangelia G Kranias
• MGI ID: MGI:3833965
• Synonyms: T17A mutant PLB, T17A PLB, Tg(Pln)22Egk, Thr¹⁷-->Ala
• Transgene: Tg(Myh6-Pln)22Egk Location: unknown
• Alliance: Tg(Myh6-Pln)22Egk page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Myh6-Pln)22Egk expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Pln (MGI:97622)

• Mutation details: The transgene contains a full length mouse cardiac phospholamban cDNA with a point mutation (ACT->GCT) that encodes alanine instead of threonine at amino acid position 17 of the protein (T17A), followed by an SV40 polyadenylation signal, under control of the cardiac specific alpha myosin heavy chain (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter. The replaced threonine normally is phosphorylated in response to stimulation of cardiac muscle beta adrenergic receptors. Northern blot analysis revealed cardiac expression of the 1.0 kb transcript from the transgene, and quantitative immunoblot analysis demonstrated expression of phospholamban protein in the hearts of transgenic mice homozygous for a null phospholamban allele. (J:66084)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:66084 Chu G, et al., A single site (Ser16) phosphorylation in phospholamban is sufficient in mediating its maximal cardiac responses to beta -agonists. J Biol Chem. 2000 Dec 8;275(49):38938-43
• All: 2 reference(s)