Ins2tm1Lef
Targeted Allele Detail
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| Symbol: |
Ins2tm1Lef |
| Name: |
insulin II; targeted mutation 1, Louis Lefebvre |
| MGI ID: |
MGI:3833432 |
| Synonyms: |
Ins2Tel7KI, Tel7KI |
| Gene: |
Ins2 Location: Chr7:142232393-142233463 bp, - strand Genetic Position: Chr7, 88.0 cM
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| Alliance: |
Ins2tm1Lef page
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| Allele Type: |
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Targeted (Null/knockout, Reporter) |
| Mutation: |
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Insertion
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Mutation details: This allele was generated by Cre recombinase mediated modification of Ins2tm1.1Nagy in ES cells. A Pgk promoter preceded by a loxP site was inserted to reactivate the promoterless loxP-neo-pA insertion residing 2.6 kb upstream of the Ins2 transcription initiation site in that mutation. The new insertion also contains a 1.6 kb array of telomere repeats and a CAG-EGFP reporter gene, which consists of the enhanced green fluorescent protein coding sequence, with a polyadenylation signal, under control of the "CAG promoter," which comprises the cytomegalovirus immediate early (CMV-IE) enhancer followed by the promoter, first exon and first intron of the chicken beta-actin gene, with the 3' splice junction sequence replaced by that of the rabbit hemoglobin beta gene. EGFP expression in the embryo is regulated by genomic imprinting from Chr 7 imprinting center 1 (IC1) and occurs only when the reporter gene is maternally inherited. In contrast, EGFP is expressed in heterozygous plancentae regardless of its maternal or paternal transmission.
(J:154092)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Ins2 Mutation: |
90 strains or lines available
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| Original: |
J:154092 Jones MJ, et al., An imprinted GFP insertion reveals long-range epigenetic regulation in embryonic lineages. Dev Biol. 2009 Dec 1;336(1):42-52 |
| All: |
2 reference(s) |
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Analysis Tools
