Gnas^tm3Kel
Targeted Allele Detail

Summary

• Symbol: Gnas^tm3Kel
• Name: GNAS complex locus; targeted mutation 3, Gavin Kelsey
• MGI ID: MGI:3830998
• Synonyms: Nesp^trun
• Gene: Gnas Location: Chr2:174126113-174188537 bp, + strand Genetic Position: Chr2, 97.89 cM, cytoband E1-H3
• Alliance: Gnas^tm3Kel page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:143477
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The Nesp transcript lies within the Gnas locus and is normally subject to maternal imprinting so that only the paternal allele is expressed in offspring. The Nesp transcript was truncated by replacing the splice donor site of the second Nesp exon with a cassette comprising the final exon, polyadenylation, and downstream sequences of the rabbit beta-globin gene and a floxed neomycin selection cassette. Chimeric founders were crossed with cre-deletor mice to excise the selection cassette. Heterozygotes were maintained by paternal transmission of the allele. Nesp transcript was not detected in the brain of neonatal heterozygotes that had maternal transmission of the mutant allele. (J:143477)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gnas Mutation: 53 strains or lines available

References

• Original: J:143477 Chotalia M, et al., Transcription is required for establishment of germline methylation marks at imprinted genes. Genes Dev. 2009 Jan 1;23(1):105-17
• All: 1 reference(s)