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Targeted Allele Detail
Symbol: Prom1tm1(cre/ERT2)Gilb
Name: prominin 1; targeted mutation 1, Richard J Gilbertson
MGI ID: MGI:3830624
Synonyms: CD133-CreERT2, Prom1C-L
Gene: Prom1  Location: Chr5:44150962-44259374 bp, - strand  Genetic Position: Chr5, 23.97 cM
Germline Transmission:  Earliest citation of germline transmission: J:144215
Parent Cell Line:  CMTI-1 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Allele Type:    Targeted (Inducible, Recombinase)
Inducer:    tamoxifen
Mutations:    Insertion, Intragenic deletion
Mutation detailsThe ATG start site of the gene locus was replaced by a cassette containing a creERT2 fusion gene, an IRES-LacZ gene, and a FRT-flanked neomycin selection cassette. Correct targeting was confirmed by PCR. The neomycin selection cassette was later removed by crossing mice with Gt(ROSA)26Sortm1(FLP1)Dym mice. Beta-gal expression was detected in the neural tube, rib, kidney, and gut of E10.5-E16.5 heterozygote embryos. Beta-gal expression was also detected in numerous adult tissues including brain, kidney, retina, pancreas, and intestine. Cre expression was also detected in brain, kidney, lung, pancreas, and intestinal epithelium of heterozygote adult mice after tamoxifen administration. Inactivation of the endogenous gene locus was confirmed by RT-PCR of adult homozygous kidney. (J:144215)
 Tissue activity of this recombinase allele
Driver: Prom1     Summary of all recombinase alleles driven by Prom1.

View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Prom1 Mutation:  44 strains or lines available
Original:  J:144215 Zhu L, et al., Prominin 1 marks intestinal stem cells that are susceptible to neoplastic transformation. Nature. 2009 Jan 29;457(7229):603-7
All:  21 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
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