Tg(Il2-EGFP)17Evr
Transgene Detail

Summary

• Symbol: Tg(Il2-EGFP)17Evr
• Name: transgene insertion 17, Ellen V Rothenberg
• MGI ID: MGI:3830507
• Synonyms: 8kbIL2p-GFP line 17, IL2p8-GFP Tg17
• Transgene: Tg(Il2-EGFP)17Evr Location: unknown
• Alliance: Tg(Il2-EGFP)17Evr page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F2

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The transgene comprises the upstream promoter/enhancer region from the mouse interleukin 2 gene extending from -8.4 kb through nucleotide +45 followed by a cDNA encoding enhanced green fluorescent protein (EGFP) and the 3' splice and polyadenylation signals from a human beta-globin gene. EGFP expression is inducible, efficient and specific to the tissue/cell types and developmental stages where IL2 normally is expressed, i.e., in peripheral T cells and in immature thymocytes and thymocytes undergoing positive selection. It was estimated by Southern blot hybridization that thirty-three copies of the transgene construct had integrated into the genome of this line. (J:89126, J:143704)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:143704 Yui MA, et al., A new regulatory region of the IL-2 locus that confers position-independent transgene expression. J Immunol. 2001 Feb 1;166(3):1730-9
• All: 2 reference(s)