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Targeted Allele Detail
Symbol: Rap1atm1Tze
Name: RAS-related protein 1a; targeted mutation 1, Tomasz Zemojtel
MGI ID: MGI:3830343
Gene: Rap1a  Location: Chr3:105727267-105801336 bp, - strand  Genetic Position: Chr3, 46.45 cM
Germline Transmission:  Earliest citation of germline transmission: J:144145
Parent Cell Line:  HM-1 (ES Cell)
Strain of Origin:  129P2/OlaHsd-Hprtb-m3
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA targeting construct was made by replacing a 420-bp region encoding the N-terminal 19 amino acids, which include conserved residues and part of the GTP-binding motif, with an internal ribosome entry site (IRES)/LacZ/ neomycin cassette. Shortened mRNAs for the locus were detected in tissue of homozygous mice. These resulted from an alternative splicing site 5' of the neomycin cassette and an alternative ATG start site that would result in a 51 amino acid truncated protein. However, no protein was detected in immunoblot analysis of thymus and spleen extracts so the authors concluded this was a null allele. LacZ staining was evident in E12.5 embryos. (J:144145)
View phenotypes and curated references for all genotypes (concatenated display).
In Mice Carrying this Mutation: 3 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Rap1a Mutation:  11 strains or lines available
Original:  J:144145 Duchniewicz M, et al., Rap1A-deficient T and B cells show impaired integrin-mediated cell adhesion. Mol Cell Biol. 2006 Jan;26(2):643-53
All:  3 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.16
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