Cdh1^tm1Mlec
Targeted Allele Detail

Summary

• Symbol: Cdh1^tm1Mlec
• Name: cadherin 1; targeted mutation 1, Marc Lecuit
• MGI ID: MGI:3822550
• Synonyms: Cdh1^E16P, E16PmEcad, Ecad^E16P, hEcad
• Gene: Cdh1 Location: Chr8:107329983-107396878 bp, + strand Genetic Position: Chr8, 53.18 cM, cytoband D
• Alliance: Cdh1^tm1Mlec page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:142843
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Intragenic deletion, Nucleotide substitutions
• Mutation details: A targeting vector was designed to change the codon within exon 4 that encodes the glutamic acid at position 16 into that of a proline (E16P). A floxed betageo selection cassette was included downstream of exon 5. Mice carrying the targeted mutation in the germline were crossed with cre transgenic mice to remove the selection cassette, leaving behind a single loxP site. Correct targeting was confirmed by genomic PCR. The amino acid substitution enhanced the affinity of the protein for the InlA surface protein expressed by Listeria monocytogenes. (J:142843)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdh1 Mutation: 171 strains or lines available

References

• Original: J:142843 Disson O, et al., Conjugated action of two species-specific invasion proteins for fetoplacental listeriosis. Nature. 2008 Oct 23;455(7216):1114-8
• All: 3 reference(s)