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H2-T23tm3Cant
Targeted Allele Detail
Nomenclature
Symbol: H2-T23tm3Cant
Name: histocompatibility 2, T region locus 23; targeted mutation 3, Harvey Cantor
MGI ID: MGI:3821721
Synonyms: Qa-1 D227K
Gene: H2-T23  Location: Chr17:36029773-36032855 bp, - strand  Genetic Position: Chr17, 18.86 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:142087
Parent Cell Line:  TC1/TC-1 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted
Mutations:    Insertion, Nucleotide substitutions
 
Mutation detailsExon 4 of the gene locus was targeted with a mutation that causes an aspartic acid to lysine exchange at amino acid position 227 (D227K). A floxed neomycin resistance cassette was also inserted downstream of exon 5 and was subsequently removed by crossing chimeric mice with a cre transgenic strain. The mutant protein was expressed on the surface of activated CD4+ T cells from homozygous mice at levels similar to the endogenous protein in wild-type mice. This mutation was predicted to disrupt binding to the CD8 coreceptor complex. (J:142087)
Phenotypes
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any H2-T23 Mutation:  5 strains or lines available
References
Original:  J:142087 Lu L, et al., Regulation of CD8+ regulatory T cells: Interruption of the NKG2A-Qa-1 interaction allows robust suppressive activity and resolution of autoimmune disease. Proc Natl Acad Sci U S A. 2008 Dec 9;105(49):19420-5
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
08/18/2015
MGI 6.0
The Jackson Laboratory