H2-T23^tm3Cant
Targeted Allele Detail

Summary

• Symbol: H2-T23^tm3Cant
• Name: histocompatibility 2, T region locus 23; targeted mutation 3, Harvey Cantor
• MGI ID: MGI:3821721
• Synonyms: Qa-1 D227K
• Gene: H2-T23 Location: Chr17:36340869-36343593 bp, - strand Genetic Position: Chr17, 18.86 cM
• Alliance: H2-T23^tm3Cant page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:142087
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 4 of the gene locus was targeted with a mutation that causes an aspartic acid to lysine exchange at amino acid position 227 (D227K). A floxed neomycin resistance cassette was also inserted downstream of exon 5 and was subsequently removed by crossing chimeric mice with a cre transgenic strain. The mutant protein was expressed on the surface of activated CD4⁺ T cells from homozygous mice at levels similar to the endogenous protein in wild-type mice. This mutation was predicted to disrupt binding to the CD8 coreceptor complex. (J:142087)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any H2-T23 Mutation: 35 strains or lines available

References

• Original: J:142087 Lu L, et al., Regulation of CD8+ regulatory T cells: Interruption of the NKG2A-Qa-1 interaction allows robust suppressive activity and resolution of autoimmune disease. Proc Natl Acad Sci U S A. 2008 Dec 9;105(49):19420-5
• All: 10 reference(s)