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Zbtb7btm2Litt
Targeted Allele Detail
Nomenclature
Symbol: Zbtb7btm2Litt
Name: zinc finger and BTB domain containing 7B; targeted mutation 2, Dan R Littman
MGI ID: MGI:3818473
Synonyms: ThPOK-GFP
Gene: Zbtb7b  Location: Chr3:89377644-89393673 bp, - strand  Genetic Position: Chr3, 39.09 cM, cytoband A1-A2
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:141519
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsExons 2 and 3 were replaced with enhanced green fluorescent protein (EGFP) coding sequence followed by the SV40 polyadenlyation signal. During the gene targeting, a loxP-flanked PGK-neomycin cassette was inserted approximately 1.1 kb upstream of the initiation codon in the sense orientation. The PGK-neomycin cassette was deleted in vivo by crossing to EIIa-cre mice and a single loxP site remains. In T lymphocytes of heterozygotes, GFP is specifically expressed in CD4 cells. In homozygotes, B220+ cells also express GFP. (J:141144, J:141519)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Zbtb7b Mutation:  19 strains or lines available
References
Original:  J:141519 Littman DR, Generation of a Zbtb7b GFP knock-in allele. MGI Direct Data Submission. 2008;
All:  8 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
01/14/2020
MGI 6.14
The Jackson Laboratory