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Cdk1tm1(Cdk2)Kald
Targeted Allele Detail
Nomenclature
Symbol: Cdk1tm1(Cdk2)Kald
Name: cyclin-dependent kinase 1; targeted mutation 1, Philipp Kaldis
MGI ID: MGI:3818306
Synonyms: Cdk1Cdk2delta12-HA, Cdk1Cdk2KI, Cdk1Cdk2N3DF4YQ5IV7I-HA
Gene: Cdk1  Location: Chr10:69335146-69352912 bp, - strand  Genetic Position: Chr10, 36.07 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:141249
Parent Cell Line:  S1B6 (ES Cell)
Strain of Origin:  129S1/SvImJ or C57BL/6J
Mutation
description
Allele Type:    Targeted (Conditional ready, Inserted expressed sequence, Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
 
Cdk1tm1(Cdk2)Kald expresses 1 gene
 
Mutation detailsA construct containing an upstream FRT site, coding sequence for the Cdk2 protein that excludes the first 12 amino acids and includes a carboxy-terminus HA tag, an IRES-beta-galactosidase gene, another FRT side and a loxP-flanked neomycin gene was used to target exon 3 of the gene locus. The insertion site maintains all the exon 3 splicing sequences of the endogenous locus. This targeted mutation resulted in a transcript that fused the 5'UTR encoded by exon 1 and the ATG start codon plus 11 amino acids encoded by exon 2 to the coding sequence of the inserted gene. The first 12 amino acids of the endogenous gene and the knock-in are very similar (they differ by four amino acids- N3D, F4Y, Q5I and V7I), suggesting that this region would not affect the Cdk2 knock-in properties. Lysates of mouse embryonic fibroblasts isolated from heterozygote mice indicated that the knock-in Cdk2 was expressed and displayed kinase activity similar to that of endogenous Cdk2. In addition, extensive Beta-galactosidase staining was observed in the spermatocytes of heterozygous mice. (J:141249)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Cdk1 Mutation:  16 strains or lines available
References
Original:  J:141249 Satyanarayana A, et al., Genetic substitution of Cdk1 by Cdk2 leads to embryonic lethality and loss of meiotic function of Cdk2. Development. 2008 Oct;135(20):3389-400
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
02/16/2021
MGI 6.16
The Jackson Laboratory