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Hprt1tm1(Nfkb1-EGFP)Cjo
Targeted Allele Detail
Summary
Symbol: Hprt1tm1(Nfkb1-EGFP)Cjo
Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Christian Jobin
MGI ID: MGI:3815189
Synonyms: cis-NF-kappaBEGFP, NF-kappaBEGFP, NFkappaBEGFP
Gene: Hprt1  Location: ChrX:52077014-52110536 bp, + strand  Genetic Position: ChrX, 29.31 cM, cytoband A6
Alliance: Hprt1tm1(Nfkb1-EGFP)Cjo page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:92020
Parent Cell Line:  BK4 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
 
Mutation detailsThree NF-kappa B response elements derived from the 5' LTR of the HIV genome and a minimal c-fos promoter were placed upstream of a EGFP reporter gene. This reporter construct was inserted upstream of genomic sequence containing the promoter and first two exons of the Hprt gene locus that is deleted in BK ES cells. Correct recombination rescued the Hprt defect, ensuring that only a single copy of the NF-kappaB reporter construct integrated into the Hprt locus. GFP positive mononuclear cells are found in both male and female mice. (J:92020)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Hprt1 Mutation:  1272 strains or lines available
References
Original:  J:92020 Magness ST, et al., In vivo pattern of lipopolysaccharide and anti-CD3-induced NF-kappa B activation using a novel gene-targeted enhanced GFP reporter gene mouse. J Immunol. 2004 Aug 1;173(3):1561-70
All:  18 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/12/2024
MGI 6.23
The Jackson Laboratory