Trp53^tm1Kasa
Targeted Allele Detail

Summary

• Symbol: Trp53^tm1Kasa
• Name: transformation related protein 53; targeted mutation 1, Kanaga Sabapathy
• MGI ID: MGI:3811714
• Synonyms: p53^R246S
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm1Kasa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:139780
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Dominant negative, Humanized sequence)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A loxP site flanked neomycin resistance gene cassette was inserted into intron 6 and exon 7 was replaced with one in which nucleotide substitutions (CGA-to-TCT) resulted in an amino acid substitution of arginine with serine at position 246 (p.R246S). Transient cre expression was used to remove the neo cassette. (J:139780)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 237 strains or lines available

References

• Original: J:139780 Lee MK, et al., The R246S hot-spot p53 mutant exerts dominant-negative effects in embryonic stem cells in vitro and in vivo. J Cell Sci. 2008 Jun 1;121(Pt 11):1899-906
• All: 2 reference(s)