Tg(IGH@yH2CM)2Amgn
Transgene Detail

Summary

• Symbol: Tg(IGH@yH2CM)2Amgn
• Name: transgene insertion 2, Amgen
• MGI ID: MGI:3810127
• Synonyms: yH2CM
• Transgene: Tg(IGH@yH2CM)2Amgn Location: unknown
• Alliance: Tg(IGH@yH2CM)2Amgn page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:139792
• Parent Cell Line: E14.1TG3B1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(IGH@yH2CM)2Amgn expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  human	IGH (3492)

• Mutation details: A yeast artificial chromosome (YAC) was constructed from human germline sequence of the immunoglobulin heavy chain gene cluster. This YAC consisted of 66 V-heavy gene segments, all 30 D gene segments, all 6 J segments, the C-mu, and the C-delta gene segments from the human locus. Downstream of this, the mouse sequence encoding the class switch recombination recognition site of gamma1 heavy chain (from the Ighg1 gene) replaced the equivalent human sequence. Downstream of this, the human exons encoding the constant region of gamma4 heavy chain were placed. A murine enhancer element were located at the 3' end of the construct. Southern blot analysis confirmed that the YAC inserted into ES cells without any recombination events. Two transgenic lines were created with line 1 having the highest expression of antibodies containing human IgG4 after immunization. (J:139792)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

The Xenomouse H2CM strain contains this allele along with the Igh-J^tm1Jkbv, Igk-C^tm1Jkbv, Tg(IGK@yK2)J23.1Jkbv alleles.

References

• Original: J:139792 Green LL, et al., Transgenic animals for producing specific isotypes of human antibodies via non-cognate switch regions. MGI Direct Data Submission. 2008
• All: 1 reference(s)