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Transgene Detail
Symbol: Tg(Foxp3-EGFP/cre)1cJbs
Name: transgene insertion 1c, Jeffrey Bluestone
MGI ID: MGI:3809724
Synonyms: FoxP3-GFP-hCre, FoxP3-GFP-hCre BAC Tg
Transgene: Tg(Foxp3-EGFP/cre)1cJbs  Location: unknown  
Strain of Origin:  NOD/ShiLt
Transgene Type:    Transgenic (Recombinase)
Mutation:    Insertion
Mutation detailsAn EGFP-h-Cre cDNA (GFP fused to a codon-optimized, "humanized" cre recombinase sequence) was inserted directly downstream of the Foxp3 ATG start codon by homologous recombination in a 188-kb C57BL/6 BAC clone (RP23-143D8) containing the entire Foxp3 gene. The purified BAC was injected into pronuclei of nonobese, diabetic mouse embryos. Several founders were generated. GFP expression and lack of functional Foxp3 protein, as indicated by inability of the transgene to rescue Foxp3 null mice were analyzed and line 1 was established. Functional cre activity was assayed by breeding mice to Gt(ROSA)26Sortm1(EYFP)Cos reporter mice, and high specificity of recombination was detected in the lymph nodes and spleen. This is the second of two lines derived from founder 1. Cre mediated recombination and GFP expression is less than in line 1a. (J:138808)
Driver: Foxp3     Summary of all recombinase alleles driven by Foxp3.

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Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Original:  J:157603 Bluestone J, A low expressing line derived from the same founder as the previously reported Tg(Foxp3-EGFP/cre)1aJbs high expressing transgene line. MGI Direct Data Submission. 2010;
All:  31 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
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