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Gt(ROSA)26Sortm14(CAG-tdTomato)Hze
Targeted Allele Detail
Nomenclature
Symbol: Gt(ROSA)26Sortm14(CAG-tdTomato)Hze
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 14, Hongkui Zeng
MGI ID: MGI:3809524
Synonyms: Ai14, Ai4, Gt(ROSA)26Sortm14(CAT-tdTomato)Hze, Gt(ROSA)26Sortm9.1(CAG-tdTomato)Hze, R26-RFP, R26RT, R26Tom, Rosa26Tdt, Rosa26-tdTomato, Rosa-CAG-LSL-tdTomato-WPRE::deltaNeo, Rosa-LSL.dTm, Rosa-lsl-tdTomato, STOP-RFP, tdTomato, Tomatoflox
Gene: Gt(ROSA)26Sor  Location: Chr6:113067428-113077333 bp, - strand  Genetic Position: Chr6, 52.73 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:155793
Parent Cell Line:  G4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Mutation
description
Allele Type:    Targeted (Conditional ready, Reporter)
Mutation:    Insertion
 
Mutation detailsThe Rosa-CAG-LSL-tdTomato-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), tdTomato sequence (a non-oligomerizing DsRed fluorescent protein variant with a 12 residue linker fusing two copies of the protein (tandem dimer)), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. phiC31-recombination of Gt(ROSA)26Sortm9(CAG-tdTomato)Hze removed the neomycin resistance cassette. (J:155793)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Expression
In Mice Carrying this Mutation: 3 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  393 strains or lines available
References
Original:  J:155793 Madisen L, et al., A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci. 2010 Jan;13(1):133-40
All:  435 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
11/14/2017
MGI 6.11
The Jackson Laboratory