Bmi1^tm1Ilw
Targeted Allele Detail

Summary

• Symbol: Bmi1^tm1Ilw
• Name: Bmi1 polycomb ring finger oncogene; targeted mutation 1, Irving L Weissman
• MGI ID: MGI:3806975
• Synonyms: Bmi-1^GFP, Bmi1^tm1Nhsn
• Gene: Bmi1 Location: Chr2:18681953-18691440 bp, + strand Genetic Position: Chr2, 12.9 cM
• Alliance: Bmi1^tm1Ilw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:138039
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: Exon 2 was replaced with an in frame EGFP cassette, exon 2 and floxed neo cassette. Transient cre expression in ES cells was used to remove the neo cassette. Expression of the EGFP precludes expression of the endogenous gene. (J:138039)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Bmi1 Mutation: 33 strains or lines available

References

• Original: J:138039 Hosen N, et al., Bmi-1-green fluorescent protein-knock-in mice reveal the dynamic regulation of bmi-1 expression in normal and leukemic hematopoietic cells. Stem Cells. 2007 Jul;25(7):1635-44
• All: 16 reference(s)