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Targeted Allele Detail
Symbol: Aff1tm1Afst
Name: AF4/FMR2 family, member 1; targeted mutation 1, A Francis Stewart
MGI ID: MGI:3806110
Synonyms: Af4-
Gene: Aff1  Location: Chr5:103692374-103855322 bp, + strand  Genetic Position: Chr5, 50.45 cM, cytoband E
Germline Transmission:  Earliest citation of germline transmission: J:138283
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
Mutation detailsAn Engrailed-2 splice acceptor was fused to the encephalomyocarditis virus internal ribosomal entry site (IRES) and place upstream of a lacZ-neomycin fusion reporter that had been modified by the insertion of a Tn903 promoter between the lacZ and the neo. This was followed by an SV40 polyadenylation signal and a Hygro reporter cassette under the control of an SV40 promoter. This construct was flanked by loxP sites and targeted into intron 3. (J:138283)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Aff1 Mutation:  273 strains or lines available
This allele was produced as part of a proof of principle for a targeted gene trap scheme.
Original:  J:138283 Testa G, et al., A reliable lacZ expression reporter cassette for multipurpose, knockout-first alleles. Genesis. 2004 Mar;38(3):151-8
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.16
The Jackson Laboratory