Aff1^tm1Afst
Targeted Allele Detail

Summary

• Symbol: Aff1^tm1Afst
• Name: AF4/FMR2 family, member 1; targeted mutation 1, A Francis Stewart
• MGI ID: MGI:3806110
• Synonyms: Af4^-
• Gene: Aff1 Location: Chr5:103840307-104003188 bp, + strand Genetic Position: Chr5, 50.45 cM, cytoband E
• Alliance: Aff1^tm1Afst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:138283
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: An Engrailed-2 splice acceptor was fused to the encephalomyocarditis virus internal ribosomal entry site (IRES) and place upstream of a lacZ-neomycin fusion reporter that had been modified by the insertion of a Tn903 promoter between the lacZ and the neo. This was followed by an SV40 polyadenylation signal and a Hygro reporter cassette under the control of an SV40 promoter. This construct was flanked by loxP sites and targeted into intron 3. (J:138283)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Aff1 Mutation: 315 strains or lines available

Notes

This allele was produced as part of a proof of principle for a targeted gene trap scheme.

References

• Original: J:138283 Testa G, et al., A reliable lacZ expression reporter cassette for multipurpose, knockout-first alleles. Genesis. 2004 Mar;38(3):151-8
• All: 1 reference(s)