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Tg(Fabp1-GH1)10Bir
Transgene Detail
Nomenclature
Symbol: Tg(Fabp1-GH1)10Bir
Name: transgene insertion 10, Edward H Birkenmeier
MGI ID: MGI:3800339
Synonyms: L-FABP(-596 to +21)/hGH transgene, short promoter L-FABP-hGH transgene, Tg(L-FABP, S-GH)10Bir
Transgene: Tg(Fabp1-GH1)10Bir  Location: unknown  
Transgene
origin
Strain of Origin:  (C57BL/6J x LT/Sv)F1
Transgene
description
Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence)
Mutation:    Insertion
 
Tg(Fabp1-GH1)10Bir expresses 1 gene
 
Mutation detailsThis transgene comprises a human growth hormone gene whose 5' regulatory elements have been replaced by nucleotides -596 to +21 from the rat liver fatty-acid binding protein gene. Mice of this line (from founder G0#19) have ~800 transgene copies per haploid genome, as measured by densitometric analysis of Southern blots. Expression of human growth hormone mRNA, determined by dot-blot analysis, correlates generally with that of endogenous mouse Fabp1 mRNA in liver and intestine, including a proximal-to-distal declining expression gradient in the small intestine (with somewhat higher hGH expression in the ileum); neither is expressed in several other tissues examined, but hGH, unlike Fabp1, is expressed in cecum, colon and kidneys of transgenic mice. This "ectopic" expression is suppressed by more 5' regulatory sequences present in similar transgenes with a longer Fabp1 promoter (See Tg(Fabp1-GH1)5Bir). (J:109939)
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:109939 Sweetser DA, et al., Mechanisms underlying generation of gradients in gene expression within the intestine: an analysis using transgenic mice containing fatty acid binding protein-human growth hormone fusion genes. Genes Dev. 1988 Oct;2(10):1318-32
All:  5 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/16/2021
MGI 6.17
The Jackson Laboratory