Tg(Pcp2-PKCI)1Cidz
Transgene Detail

Summary

• Symbol: Tg(Pcp2-PKCI)1Cidz
• Name: transgene insertion 1, CI De Zeeuw
• MGI ID: MGI:3797386
• Synonyms: L7-PKCI
• Transgene: Tg(Pcp2-PKCI)1Cidz Location: unknown
• Alliance: Tg(Pcp2-PKCI)1Cidz page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A minigene containing a PKC inhibitor was made by hybridization of two complementary synthetic oligonucleotides, each 50 bases in length, producing a double-stranded product with overhangs compatible with BamHI restriction sites. The minigene, is expressed under the control of a Purkinje cell specific promoter, and encodes a 13 amino acid peptide (not including the start methionine) corresponding in sequence to PKC[19-31] slightly truncated version of the pseudosubstrate domain of PKC, PKC[19-36] This version has been demonstrated in vitro to be a more potent inhibitor of PKC (by 60%) than the longer version, but both were shown by biochemical assay to block selectively the complete range of isotypes present in PKC purified from whole brain. Expression of the inhibitory peptide in Purkinje cells was confirmed by an electrophysiological assay of a phorbol ester-dependent effect that is attenuated in cerebellar cultures prepared from L7-PKCI mice. (J:108739)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:108739 De Zeeuw CI, et al., Expression of a protein kinase C inhibitor in Purkinje cells blocks cerebellar LTD and adaptation of the vestibulo-ocular reflex. Neuron. 1998 Mar;20(3):495-508
• All: 14 reference(s)