Tg(CAG-EGFP,-ALPP)2.6Ggc
Transgene Detail

Summary

• Symbol: Tg(CAG-EGFP,-ALPP)2.6Ggc
• Name: transgene insertion 2.6, Giacomo Consalez
• MGI ID: MGI:3797263
• Synonyms: eGFP-F-IRES-hPLAP, piGAP
• Transgene: Tg(CAG-EGFP,-ALPP)2.6Ggc Location: unknown
• Alliance: Tg(CAG-EGFP,-ALPP)2.6Ggc page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The transgene was designed with a CMV enhancer/chicken beta-actin promoter (from pCAGGS vector) and loxP-flanked STOP-polyA sequence upstream of a farnesylated Enhanced Green Fluorescent Protein coding sequence (eGFP-F; from pEGFP-F plasmid (Clontech), eGFP fused to the carboxyl-terminal 20 amino acids of the human c-Ha-Ras protein, which targets GFP to the cytoplasmic side of the plasma membrane), internal ribosomal entry site (IRES), human Placental Alkaline Phosphatase cDNA sequence (PLAP or ALPP; from the pGTO plasmid), and SV40 polyA signal. (J:136874)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:136874 Badaloni A, et al., Transgenic mice expressing a dual, CRE-inducible reporter for the analysis of axon guidance and synaptogenesis. Genesis. 2007 Jun;45(6):405-12
• All: 1 reference(s)