Tg(PGK-GUS*E540A)55Sly
Transgene Detail

Summary

• Symbol: Tg(PGK-GUS*E540A)55Sly
• Name: transgene insertion 55, William S Sly
• MGI ID: MGI:3795176
• Synonyms: MPS VII/E540A^Tg, Tg hGUS E540A
• Transgene: Tg(PGK-GUS*E540A)55Sly Location: unknown
• Alliance: Tg(PGK-GUS*E540A)55Sly page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(PGK-GUS*E540A)55Sly expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  GUSB (2990)

  Gusb	(MGI:95872)

  Transgene codes for a protein with a glutamic acid to alanine mutation.

• Mutation details: The 2.2-kb human GUS E540A cDNA is expressed under the control of a the 0.54-kb mouse phosphoglycerate kinase (PGK) promoter and a 3' element including the rabbit beta-globin intron and an SV40 poly(A) signal. (J:67876)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:67876 Sly WS, et al., Active site mutant transgene confers tolerance to human beta -glucuronidase without affecting the phenotype of MPS VII mice. Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2205-10
• All: 3 reference(s)