Ppp4c^tm1.2Htn
Targeted Allele Detail

Summary

• Symbol: Ppp4c^tm1.2Htn
• Name: protein phosphatase 4, catalytic subunit; targeted mutation 1.2, Shinji Hirotsune
• MGI ID: MGI:3794751
• Synonyms: PP4c^-, Tbx6/Ppp4c^tm1.2Htn
• Gene: Ppp4c Location: Chr7:126385038-126391668 bp, - strand Genetic Position: Chr7, 69.25 cM
• Alliance: Ppp4c^tm1.2Htn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:134682
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intergenic deletion
• Ppp4c^tm1.2Htn involves 2 genes/genome features (Tbx6, Ppp4c) View all
• Mutation details: A loxP site was inserted in between exons 4 and 5. A floxed neomycin resistance cassette was inserted downstream of exon 8 and also replaced the codons of the last 4 amino acids of Tbx6. Cre-mediated recombination removed the selection cassette. Northern blot analysis confirmed normal Ppp4c transcript expression in embryo. RT-PCR confirmed normal Tbx6 transcript expression. Cre-mediated recombination removed the selection cassette and exons 5 through 8. (J:134682)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ppp4c Mutation: 40 strains or lines available

References

• Original: J:134682 Toyo-oka K, et al., Protein phosphatase 4 catalytic subunit regulates Cdk1 activity and microtubule organization via NDEL1 dephosphorylation. J Cell Biol. 2008 Mar 24;180(6):1133-47
• All: 1 reference(s)