Tg(H2-Ea*deltaY)54Dim
Transgene Detail

Summary

• Symbol: Tg(H2-Ea*deltaY)54Dim
• Name: transgene insertion 54, Diane Mathis
• MGI ID: MGI:3793482
• Synonyms: Tg(dY301)3Strasbourg, Tg(dY301,54)3Strasbourg, WEdeltaY301, WEdeltaY301-54
• Transgene: Tg(H2-Ea*deltaY)54Dim Location: unknown
• Alliance: Tg(H2-Ea*deltaY)54Dim page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(H2-Ea*deltaY)54Dim expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	H2-I (MGI:95903)

• Mutation details: A 9 kb fragment of the H2-Ea(k) from the A/J mouse (clone lambdaA/J p34.13) was modified at 3 positions creating the WE32 construct (modified H2-Ea). This was used as the basis for a mutant H2-Ea gene having a deletion of the Y box (from position -61 to position -48). The resulting 8 kb transgenic construct WEdeltaY301 was injected into (C57BL/6 x SJL)F2 embryo pronuclei. Multiple lines - 22, 45, 54, 58, and 59, were derived, and 54 was donated to The Jackson Laboratory. S1 nuclease mapping shows that little H2-Ea mRNA is expressed in the spleen. Initiation of transcripts is aberrant. (J:135138)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:135138 Dorn A, et al., Conserved major histocompatibility complex class II boxes--X and Y--are transcriptional control elements and specifically bind nuclear proteins. Proc Natl Acad Sci U S A. 1987 Sep;84(17):6249-53
• All: 1 reference(s)