Chst8^tm1.1Jub
Targeted Allele Detail

Summary

• Symbol: Chst8^tm1.1Jub
• Name: carbohydrate sulfotransferase 8; targeted mutation 1.1, Jacques U Baenziger
• MGI ID: MGI:3793437
• Synonyms: GalNAc-4-ST1^-
• Gene: Chst8 Location: Chr7:34373893-34512136 bp, - strand Genetic Position: Chr7, 20.53 cM, cytoband B3-B5
• Alliance: Chst8^tm1.1Jub page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:135143
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neo cassette was inserted downstream of exon 4 and an additional loxP site was inserted upstream of exon 3. Cre-mediate recombination in ES cells removed both exon 3, exon 4 and the neo cassette. The absence of transcript was confirmed by RT-PCR and enzymatic assays revealed a less than 5% of wild-type activity level. (J:135143)

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Chst8 Mutation: 84 strains or lines available

References

• Original: J:135143 Mi Y, et al., Ablation of GalNAc-4-sulfotransferase-1 enhances reproduction by altering the carbohydrate structures of luteinizing hormone in mice. J Clin Invest. 2008 May;118(5):1815-24
• All: 1 reference(s)