En1^tm8.1Alj
Targeted Allele Detail

Summary

• Symbol: En1^tm8.1Alj
• Name: engrailed 1; targeted mutation 8.1, Alexandra L Joyner
• MGI ID: MGI:3789091
• Synonyms: En1^flox
• Gene: En1 Location: Chr1:120530246-120535719 bp, + strand Genetic Position: Chr1, 52.74 cM, cytoband C2-qter
• Alliance: En1^tm8.1Alj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:134516
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting construct was designed to insert an frt-flanked neomycin cassette and loxP site upstream, and a loxP site downstream of the second exon of the targeted gene. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with Black Swiss mice (to avoid rescue of the En1 mutant phenotype by the C57BL/6 background). The resulting En1flox-neo mice were then bred to 129 mice constitutively expressing Flp recombinase to remove the Frt-flanked neo cassette, generating En1flox offspring. (J:134516)

Expression

In Mice Carrying this Mutation:	5 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any En1 Mutation: 32 strains or lines available

References

• Original: J:134516 Sgaier SK, et al., Genetic subdivision of the tectum and cerebellum into functionally related regions based on differential sensitivity to engrailed proteins. Development. 2007 Jun;134(12):2325-35
• All: 15 reference(s)