Thsd1^tm1Hina
Targeted Allele Detail

Summary

• Symbol: Thsd1^tm1Hina
• Name: thrombospondin, type I, domain 1; targeted mutation 1, Hiromitsu Nakauchi
• MGI ID: MGI:3777581
• Synonyms: Tmtsp^Venus
• Gene: Thsd1 Location: Chr8:22717329-22751350 bp, + strand Genetic Position: Chr8, 11.16 cM, cytoband A3
• Alliance: Thsd1^tm1Hina page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:132871
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A cassette containing a splice donor, splice acceptor, Venus and a poly-adenylation signal followed by a floxed neo cassette was inserted into exon 2 replacing 12 base pairs of sequencing including the first two ATGs. The expression of Venus was confirmed by immunofluorescence. (J:132871)

Expression

In Mice Carrying this Mutation:

3 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Thsd1 Mutation: 48 strains or lines available

References

• Original: J:132871 Takayanagi S, et al., Genetic marking of hematopoietic stem and endothelial cells: identification of the Tmtsp gene encoding a novel cell surface protein with the thrombospondin-1 domain. Blood. 2006 Jun 1;107(11):4317-25
• All: 2 reference(s)