Tg(CD2-Ahr*,CD2-GFP)AKno
Transgene Detail

Summary

• Symbol: Tg(CD2-Ahr*,CD2-GFP)AKno
• Name: transgene insertion A, Keiko Nohara
• MGI ID: MGI:3776513
• Synonyms: Tg(hCD2-CA-AhR/GFP)A
• Transgene: Tg(CD2-Ahr*,CD2-GFP)AKno Location: unknown
• Alliance: Tg(CD2-Ahr*,CD2-GFP)AKno page

Transgene origin

• Strain of Origin: C57BL/6J x DBA/2

Transgene description

• Transgene Type: Transgenic (Constitutively active, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: This transgene was generated by coinjection of two transgene constructs, one expressing a constitutively active mutant mouse aryl hydrocarbon receptor and the other expressing green fluorescent protein, both under control of the T-cell lineage-specific human CD2 promoter. It was estimated by Southern blot analysis that Line A has two copies of the cointegrated transgenes. Transgene expression was detected by RT-PCR in thymus, spleen and lung and to a very minor extent in kidney. Flow cytometry analysis of GFP expression revealed a broader peak of fluorescence for thymocytes than for splenocytes; splenic Cd4⁺ and CD8⁺ T cells express GFP, while splenic B cells do not. (J:97720)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:97720 Nohara K, et al., Constitutively active aryl hydrocarbon receptor expressed specifically in T-lineage cells causes thymus involution and suppresses the immunization-induced increase in splenocytes. J Immunol. 2005 Mar 1;174(5):2770-7
• All: 3 reference(s)