Fstl4^tm1Mno
Targeted Allele Detail

Summary

• Symbol: Fstl4^tm1Mno
• Name: follistatin-like 4; targeted mutation 1, Masaharu Noda
• MGI ID: MGI:3775825
• Synonyms: SPIG1^gfp
• Gene: Fstl4 Location: Chr11:52655461-53079365 bp, + strand Genetic Position: Chr11, 31.86 cM
• Alliance: Fstl4^tm1Mno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:132129
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A GFP-neo cassette with sequence encoding a 14 amino acid linker was inserted in frame into exon 2 replacing most of it. Only the first 4 amino acids of exon 2, the first coding exon, are expressed. The presence of the fusion transcript and protein was confirmed by RT-PCR and immunohistochemistry, respectively. The absence of the endogenous transcript was confirmed by RT-PCR. This is a null allele. (J:132129)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fstl4 Mutation: 48 strains or lines available

References

• Original: J:132129 Yonehara K, et al., Expression of SPIG1 Reveals Development of a Retinal Ganglion Cell Subtype Projecting to the Medial Terminal Nucleus in the Mouse. PLoS One. 2008;3(2):e1533
• All: 7 reference(s)