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Eif2ak4tm1.1Dron
Targeted Allele Detail
Nomenclature
Symbol: Eif2ak4tm1.1Dron
Name: eukaryotic translation initiation factor 2 alpha kinase 4; targeted mutation 1.1, David Ron
MGI ID: MGI:3774318
Synonyms: Eif2ak4tm1.1Munn, GCN2cko, GCN2conditional
Gene: Eif2ak4  Location: Chr2:118388618-118475234 bp, + strand  Genetic Position: Chr2, 59.4 cM, cytoband E5
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:129841
Parent Cell Line:  W4 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
 
Mutation detailsExon 12 was flanked by loxP sites by inserting a loxP sequence into the EcorRI site in intron 11, and a floxed neomycin resistance cassette into the NheI site of intron 12. After homologous recombination, Cre was transiently expressed and ES cells were selected that had the neomycin cassette removed. Geneotypes were confirmed by PCR. The floxed exon 12 encodes a region of the kinase domain required for ATP binding. In addition, removal of this exon leads to a frameshift mutation with multiple stop codons. (J:129841)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Eif2ak4 Mutation:  49 strains or lines available
References
Original:  J:129841 Maurin AC, et al., The GCN2 kinase biases feeding behavior to maintain amino acid homeostasis in omnivores. Cell Metab. 2005 Apr;1(4):273-7
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
09/17/2019
MGI 6.14
The Jackson Laboratory