Tcra^{tm1(Tcra)Rsky}
Targeted Allele Detail

Summary

• Symbol: Tcra^{tm1(Tcra)Rsky}
• Name: T cell receptor alpha chain; targeted mutation 1, Klaus Rajewsky
• MGI ID: MGI:3773838
• Synonyms: HYalpha^IF
• Gene: Tcra Location: Chr14:52665424-54461655 bp, + strand Genetic Position: Chr14, cytoband D1-D2

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81564
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: This allele of the gene locus was constructed in two steps. A loxP site flanked neomycin resistance gene cassette was inserted upstream of the Trav6d-3 (Valpha6/Vdelta3) segment located at the boundary of the Trav and Trdv gene segments. After targeting, the neo cassette was removed by transiently expressing Cre recombinase to leave in its place a single loxP site. ES cells were then targeted downstream of the Tcrd locus to replace the Traj57 (Jalpha57) segment with a recombined Trav9-2/Jalpha57 segment. Upstream of the recombined element a neo cassette and a loxP site were inserted. ES cells were screened by Southern blotting for targeting events that occurred in cis. These mice have an intact Tcrd locus that is flanked by loxP sites and have a recombined Valpha-Jalpha segment downstream that partly encodes a T cell receptor that recognizes the HY male antigen. (J:81564)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcra Mutation: 102 strains or lines available

References

• Original: J:81564 Buch T, et al., Failure of HY-specific thymocytes to escape negative selection by receptor editing. Immunity. 2002 May;16(5):707-18
• All: 1 reference(s)