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Targeted Allele Detail
Symbol: Pou5f1tm1Jae
Name: POU domain, class 5, transcription factor 1; targeted mutation 1, Rudolf Jaenisch
MGI ID: MGI:3771368
Synonyms: Oct4-IRES-GFPneo, Oct4-neo
Gene: Pou5f1  Location: Chr17:35816929-35821674 bp, + strand  Genetic Position: Chr17, 18.69 cM
Germline Transmission:  Earliest citation of germline transmission: J:130250
Parent Cell Line:  v6.5 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
Mutation detailsThe Oct4-IRES-GFPneo targeting vector was designed to insert an IRES-GFPneo fusion cassette downstream of exon 5. While sufficient neomycin-resistance activity is observed in embryonic stem cells, no visible GFP fluorescence is reported. (J:130250)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pou5f1 Mutation:  79 strains or lines available
When treated with specific transcription factors (Oct4, Sox2, c-Myc and Klf4), some Oct4-neo murine embryonic fibroblasts (MEFs) have the properties of induced pluripotent stem (iPS) cells. Such iPS cells have the DNA methylation, gene expression and chromatin state of embryonic stem cells and can form viable chimeras, contribute to the germ line, and generate live late-term embryos when injected into tetraploid blastocysts.
Original:  J:130250 Wernig M, et al., In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state. Nature. 2007 Jul 19;448(7151):318-24
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
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