Abcg2^tm2Bsor
Targeted Allele Detail

Summary

• Symbol: Abcg2^tm2Bsor
• Name: ATP binding cassette subfamily G member 2 (Junior blood group); targeted mutation 2, Brian P Sorrentino
• MGI ID: MGI:3768641
• Synonyms: Abcg2^GFP
• Gene: Abcg2 Location: Chr6:58561476-58669436 bp, + strand
• Alliance: Abcg2^tm2Bsor page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:128682
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: A loxP-flanked neomycin selection cassette and an IRES-GFP reporter gene were inserted immediately downstream of the stop codon and upstream of the endogenous polyadenylation signal sequence. Targeting took place in ES cells homozygous for the Tg(Prm-cre)70Og transgene, which expresses Cre recombinase in male germ cells. Cre-mediated excision subsequently removed the neomycin cassette upon transmission from male chimeric mice. Reporter gene expression was confirmed by immunohistochemistry on intestine and kidney from homozygotes. (J:128682)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Abcg2 Mutation: 75 strains or lines available

References

• Original: J:128682 Tadjali M, et al., Prospective isolation of murine hematopoietic stem cells by expression of an Abcg2/GFP allele. Stem Cells. 2006 Jun;24(6):1556-63
• All: 3 reference(s)