Tg(DBH-RET*M918T)CH7720Rpk
Transgene Detail

Summary

• Symbol: Tg(DBH-RET*M918T)CH7720Rpk
• Name: transgene insertion CH7720, Raj P Kapur
• MGI ID: MGI:3767822
• Synonyms: DbetaH-RET^MEN2B, `low'
• Transgene: Tg(DBH-RET*M918T)CH7720Rpk Location: unknown
• Alliance: Tg(DBH-RET*M918T)CH7720Rpk page

Transgene origin

• Strain of Origin: (DBA x C57BL/6)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(DBH-RET*M918T)CH7720Rpk expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  RET (5979)

  Ret	(MGI:97902)

  Transgene codes for a protein with a methionine to threonine mutation.

• Mutation details: A human cDNA encoding the short isoform (1072 amino acids) of RET was mutated by PCR to introduce the RET MEN2B mutation (Met918Thr). This mutated cDNA was inserted downstream of a human DBH promoter (5.8 kb). Between the transcriptional start site and the mutated cDNA, the construct contains the first intron of the rat preproinsulin gene (+13 +183 nt). A polyadenylation site from the murine protamine 1 gene was inserted at the 3' end. This transgenic line has a copy number of 4. (J:108163)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:108163 Sweetser DA, et al., Ganglioneuromas and renal anomalies are induced by activated RET(MEN2B) in transgenic mice. Oncogene. 1999 Jan 28;18(4):877-86
• All: 3 reference(s)