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Lgr5tm1(cre/ERT2)Cle
Targeted Allele Detail
Nomenclature
Symbol: Lgr5tm1(cre/ERT2)Cle
Name: leucine rich repeat containing G protein coupled receptor 5; targeted mutation 1, Hans Clevers
MGI ID: MGI:3764660
Synonyms: Lgr5-cre, Lgr5CREER, Lgr5-creERT, Lgr5-CreERT2-IRES-EGFP, Lgr5-EGFP, Lgr5 EGFP-IRES-creERT2, Lgr5-EGFP-IRES-creERT2, Lgr5tm1(cre/ESR1)Cle
Gene: Lgr5  Location: Chr10:115450314-115587780 bp, - strand  Genetic Position: Chr10, 63.51 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:127123
Parent Cell Line:  IB10/E14IB10 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Inducible, Recombinase, Reporter)
Inducer:    tamoxifen
Mutation:    Insertion
 
Mutation detailsAn EGFP-IRES-creERT2 cassette was inserted into exon 1 of the gene along with a loxP-flanked neomycin cassette. The neomycin gene was then excised in vivo by crossing with cre-deleter strain. GFP expression is driven by the endogenous promoter. Cre expression is induced in Lgr5 expressing cells by tamoxifen treatment. (J:127123)
Recombinase
activity
Activity:
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Driver: Lgr5     Summary of all recombinase alleles driven by Lgr5. Comparative matrix view of recombinase activities
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 4 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Lgr5 Mutation:  24 strains or lines available
References
Original:  J:127123 Barker N, et al., Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature. 2007 Oct 25;449(7165):1003-7
All:  313 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/26/2019
MGI 6.14
The Jackson Laboratory