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Transgene Detail
Symbol: Tg(CAG-Serpine1)1Dgi
Name: transgene insertion 1, David Ginsburg
MGI ID: MGI:3763900
Synonyms: PAI-1 line 1, PAI1 transgenic (actin promoter), Tg(Actb-Serpine1)1Dgi
Transgene: Tg(CAG-Serpine1)1Dgi  Location: unknown  
Strain of Origin:  (C57BL/6 x SJL)F1
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
Tg(CAG-Serpine1)1Dgi expresses 1 gene
Mutation detailsOligo-based site-directed mutagenesis was use to mutate codon 177 from AAG to ACC (K154T), and codon 342 from CAG to CTG (Q319L) in the mature mouse protein. Expression is driven by the CAG promoter (the chicken beta-actin (Actb) promoter with a CMV immediate-early enhancer). The 2 amino acid substitutions more than triple the functional half-life of the protein and expression is increased approximately 170-fold over wildtype. Quantitative PCR reveals elevated expression in brain, heart, lung, liver, spleen, intestine, kidney, fat, skin, and muscle (strongest in liver, brain, heart and kidney). Immunohistochemistry reveals expression in kidney, liver, spleen, and lung. (J:167969)
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Original:  J:167969 Fahim AT, et al., Transgenic overexpression of a stable Plasminogen Activator Inhibitor-1 variant. Thromb Res. 2009 Mar;123(5):785-92
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.16
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