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Tg(CAG-Ub*G76V/GFP)1Dant
Transgene Detail
Nomenclature
Symbol: Tg(CAG-Ub*G76V/GFP)1Dant
Name: transgene insertion 1, Nico Dantuma
MGI ID: MGI:3762797
Synonyms: Tg(ACTB-Ub*G76V/GFP)1Dant, Tg(CAG-Ub*G76V/GFP)1Dant, UbG76V-GFP, UbG76V-GFP/1, UbG76V-GFP/2, UPS reporter
Transgene: Tg(CAG-Ub*G76V/GFP)1Dant  Location: unknown  
Transgene
origin
Strain of Origin:  (CBA x C57BL/6)F1
Transgene
description
Transgene Type:    Transgenic (Reporter)
Mutation:    Insertion
 
Mutation detailsThe UbG76V-GFP transgene was designed with a chicken beta-actin promoter (and cytomegalovirus (CMV) immediate early enhancer) upstream of a green fluorescent protein (GFP) fused to a mutant ubiquitin moiety (UbG76V). Although transcripts of the UbG76V-GFP fusion gene are detected in all tissues examined, the G76V substitution prevents removal of the ubiquitin moiety leading to efficient ubiquitination and proteasomal degradation of the fusion protein (and thus no fluorescence) in these tissues. Line 1 with high expression was maintained by backcrosses to C57BL/6N mice. The high expressing line (UbG76V-GFP/1) has very low, but detectable, fluorescence expression in brain, spinal cord, and exocrine pancreatic tissue prior to administration of proteasome inhibitors. (J:127085, J:127087)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:127085 Lindsten K, et al., A transgenic mouse model of the ubiquitin/proteasome system. Nat Biotechnol. 2003 Aug;21(8):897-902
All:  12 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/30/2021
MGI 6.17
The Jackson Laboratory